high-performance liquid chromatographic analysis of sulfur mustard reaction with amino acids and proteins

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF SULFUR MUSTARD REACTION WITH AMINO ACIDS AND PROTEINS

The interaction of sulfur mustard with aminoacids and proteins has been investigated in this study. Rats were injected with sublethal doses of sulfur mustard subcutaneously and intraperitoneally. At different time intervals, plasma and urine samples were collected. The binding affinity of sulfur mustard with urinary and plasma proteins and enzymes was studied for the first time using non-i...

State-of-the-art of high-performance liquid chromatographic analysis of amino acids in physiological samples.

Nowadays, the fast and sensitive high-performance liquid chromatographic (HPLC) analysis of derivatized amino acids is a good alternative to the ion-exchange chromatography (IEC) method for plasma amino acids. However, several precautions have to be taken in order to obtain reliable data on the concentration of amino acids in physiological fluids. These include the collection, centrifugation, s...

High-performance liquid chromatographic analysis of amino acids in ackee fruit with emphasis on the toxic amino acid hypoglycin A.

High-performance liquid chromatography is used to determine the amino acid content of ripe and unripe ackee fruit. Specific emphasis is placed on the level of the toxic amino acid hypoglycin A (hyp-A) in the unripe and ripe ackee fruit and seed. Unripe samples are found to contain significantly higher quantities (P < 0.05) of hyp-A when compared with ripe samples. Uncooked unripe fruit is found...

High performance liquid chromatographic analysis of sugars in raw and processed potatoes

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM

A simple, rapid and sensitive HPLC method for the determination of theophylline (T) in human serum has been developed. An isocratic system consisting of a /-l Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5), and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal ...

Review: State-of-the-art of high-performance liquid chromatographic analysis of amino acids in physiological samples

Nowadays, the fast and sensitive high-performance liquid chromatographic I HPLC) analysis of derivatized amino acids is a good alternative to the ion-exchange chromatography (IEC) method for plasma amino acids. However, several precautions have to be taken in order to obtain reliable data on the concentration of amino acids in physiological fluids. These include the collection, centrifugation, ...